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bmp 7  (R&D Systems)


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    Structured Review

    R&D Systems bmp 7
    Bmp 7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bmp+7/Recombinant+Human+BMP-7+Protein/pm41967630-70-6-14
    Average 95 stars, based on 116 article reviews
    bmp 7 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair.
    Article Snippet: Accepted Manuscript Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair Hee Jung Kim, Min Ah.. Han, Ji Young Shin, Jeong Hwa Jeon, Seung Jin Lee, Mi Yun Yoon, Han-Jun Kim, Eun-Ji Choi, Sun Hee Do, Victor C. Yang, Huining He, Young-Il Yang PII: S0168-3659(19)30194-4 DOI: https://doi.org/10.1016/j.jconrel.2019.04.002 Reference: COREL 9717 To appear in: Journal of Controlled Release Received date: 28 September 2018 Revised date: 31 March 2019 Accepted date: 3 April 2019 Please cite this article as: H.J.. Kim, M.A.

    Article Title: Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells
    Article Snippet: Hyaluronic acid (HA, sodium salt, M w : 90–110 kDa), and p -dioxanone were purchased from Sigma-Aldrich (Shanghai, China). .. Recombinant human BMP-7 and BMP-7 ELISA kit were purchased from R&D systems (Minneapolis, MN, USA). ..

    Article Title: BMP7-induced-Pten inhibits Akt and prevents renal fibrosis.
    Article Snippet: .. 49 Animals were randomized into two groups (n=5 each) receiving vehicle (20mM histidine, 140mM NaCl, Roche) or 300g/kg recombinant human BMP-7 (R&D systems) i.p. on days 0, 2, 4, and 6. ..

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Article Title: Radiolabeling and In Vivo Imaging of Transplanted Renal Lineages Differentiated from Human Embryonic Stem Cells in Fetal Rhesus Monkeys
    Article Snippet: .. Once cells were shown with a normal karyotype and documented to express luciferase, hES cell colonies were removed from underlying feeder layers by collagenase treatment and cultured in high-glucose DMEM medium containing 10% fetal bovine serum (Hyclone, Logan, UT), 1% Penicillin–Streptomycin, 1% L-glutamine, 100 μM 1-thioglycerol (Sigma) supplemented with 0.1 μM trans-Retinoic Acid (EMD Biosciences, San Diego, CA), 10 ng/ml Activin-A (R&D Systems, Minneapolis, MN), and 50 ng/ml recombinant human BMP-7 (R&D Systems) as previously described [ 9 ]. .. 64 Cu-PTSM Radiolabeling hES cells expressing firefly luciferase were dissociated using trypsin–EDTA (Invitrogen), counted, and plated in a 96-well plate in triplicate at 1.0×10 5 , 5.0×10 4 , 2.5×10 4 , 1.3×10 4 , 6.3×10 3 , 3.1×10 3 , and 1.6×10 3 cells per well.

    Article Title: Effect of low-intensity pulsed ultrasound on bone morphogenetic protein 7-induced osteogenic differentiation of human nonunion tissue-derived cells in vitro.
    Article Snippet: lthough fracture healing is uneventful in most cases, 5% to 10% of fractures fail to heal and result in a delayed union or persistent nonunion.1,2 Autogenous bone grafting has been the standard technique for enhancing nonunion healing for many years.3 However, bone grafting has drawbacks, such as the need for an additional surgical site with its associated perioperative morbidity (eg, pain, potential infection, blood loss, and fracture),4 and limits exist with respect to the size, shape, and quantity of bone grafts available.. Other treatment techniques have thus been developed.. Biological Takaaki Koga, MD, Sang Yang Lee, MD, PhD, Takahiro Niikura, MD, PhD, Akihiro Koh, MD, PhD, Yoshihiro Dogaki, MD, Etsuko Okumachi, MD, Toshihiro Akisue, MD, PhD, Ryosuke Kuroda, MD, PhD, Masahiro Kurosaka, MD, PhD

    Article Title: Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology
    Article Snippet: .. ODM was composed of Minimum Essential Medium α (ThermoFisher Scientific, cat# 12571063) supplemented with 10% KnockOut Serum Replacement (ThermoFisher Scientific, cat# 10828028), hepatocyte growth factor (5 ng/mL) (R&D Systems, cat# 294-HG), activin A (100 ng/mL) (Sigma-Aldrich, cat# a4941), follicle stimulating hormone (1 ng/mL) (Sigma-Aldrich, cat# F4021), luteinizing hormone (1 ng/mL) (Sigma-Aldrich, cat# L5259), testosterone (1 μM) (Steraloids, cat# A6950-000), recombinant human BMP-4 (20 ng/mL) (R&D Systems, cat# 314-BP), recombinant human BMP-7 (20 ng/mL) (R&D Systems, cat# 354-BP), 3,3’,5-triodo-L-thyronine sodium (2 ng/mL) (Sigma-Aldrich, cat# T6397), l-ascorbic acid-2-glucoside (1 mM) (Matrix Scientific, cat# 092375) and 1% Penicillin-Streptomycin ( , ). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair.
    Article Snippet: Accepted Manuscript Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair Hee Jung Kim, Min Ah.. Han, Ji Young Shin, Jeong Hwa Jeon, Seung Jin Lee, Mi Yun Yoon, Han-Jun Kim, Eun-Ji Choi, Sun Hee Do, Victor C. Yang, Huining He, Young-Il Yang PII: S0168-3659(19)30194-4 DOI: https://doi.org/10.1016/j.jconrel.2019.04.002 Reference: COREL 9717 To appear in: Journal of Controlled Release Received date: 28 September 2018 Revised date: 31 March 2019 Accepted date: 3 April 2019 Please cite this article as: H.J.. Kim, M.A.

    Article Title: Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells
    Article Snippet: Hyaluronic acid (HA, sodium salt, M w : 90–110 kDa), and p -dioxanone were purchased from Sigma-Aldrich (Shanghai, China). .. Recombinant human BMP-7 and BMP-7 ELISA kit were purchased from R&D systems (Minneapolis, MN, USA). ..

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    High Molecular Weight:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Viscosity:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Picogreen Assay:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Pyrophosphate Assay:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Staining:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Cell Culture:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Article Title: Radiolabeling and In Vivo Imaging of Transplanted Renal Lineages Differentiated from Human Embryonic Stem Cells in Fetal Rhesus Monkeys
    Article Snippet: .. Once cells were shown with a normal karyotype and documented to express luciferase, hES cell colonies were removed from underlying feeder layers by collagenase treatment and cultured in high-glucose DMEM medium containing 10% fetal bovine serum (Hyclone, Logan, UT), 1% Penicillin–Streptomycin, 1% L-glutamine, 100 μM 1-thioglycerol (Sigma) supplemented with 0.1 μM trans-Retinoic Acid (EMD Biosciences, San Diego, CA), 10 ng/ml Activin-A (R&D Systems, Minneapolis, MN), and 50 ng/ml recombinant human BMP-7 (R&D Systems) as previously described [ 9 ]. .. 64 Cu-PTSM Radiolabeling hES cells expressing firefly luciferase were dissociated using trypsin–EDTA (Invitrogen), counted, and plated in a 96-well plate in triplicate at 1.0×10 5 , 5.0×10 4 , 2.5×10 4 , 1.3×10 4 , 6.3×10 3 , 3.1×10 3 , and 1.6×10 3 cells per well.

    Article Title: Effect of low-intensity pulsed ultrasound on bone morphogenetic protein 7-induced osteogenic differentiation of human nonunion tissue-derived cells in vitro.
    Article Snippet: lthough fracture healing is uneventful in most cases, 5% to 10% of fractures fail to heal and result in a delayed union or persistent nonunion.1,2 Autogenous bone grafting has been the standard technique for enhancing nonunion healing for many years.3 However, bone grafting has drawbacks, such as the need for an additional surgical site with its associated perioperative morbidity (eg, pain, potential infection, blood loss, and fracture),4 and limits exist with respect to the size, shape, and quantity of bone grafts available.. Other treatment techniques have thus been developed.. Biological Takaaki Koga, MD, Sang Yang Lee, MD, PhD, Takahiro Niikura, MD, PhD, Akihiro Koh, MD, PhD, Yoshihiro Dogaki, MD, Etsuko Okumachi, MD, Toshihiro Akisue, MD, PhD, Ryosuke Kuroda, MD, PhD, Masahiro Kurosaka, MD, PhD

    Pore Size:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Positron Emission Tomography:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    ALP Assay:

    Article Title: A combinatorial approach towards achieving an injectable, self-contained, phosphate-releasing scaffold for promoting biomineralization in critical size bone defects.
    Article Snippet: An injectable, guanosine 50-diphosphate (GDP)-crosslinked chitosan sponge was investigated as a drug delivery system (DDS) for accelerating biomineralization in critical size bone defects (CSBDs).. Two approaches were examined both individually, and in combination, in order to achieve this goal.. The first approach involved the encapsulation and release of Bone Morphogenetic Protein 7 (BMP-7), a powerful mineralization stimulant.

    Luciferase:

    Article Title: Radiolabeling and In Vivo Imaging of Transplanted Renal Lineages Differentiated from Human Embryonic Stem Cells in Fetal Rhesus Monkeys
    Article Snippet: .. Once cells were shown with a normal karyotype and documented to express luciferase, hES cell colonies were removed from underlying feeder layers by collagenase treatment and cultured in high-glucose DMEM medium containing 10% fetal bovine serum (Hyclone, Logan, UT), 1% Penicillin–Streptomycin, 1% L-glutamine, 100 μM 1-thioglycerol (Sigma) supplemented with 0.1 μM trans-Retinoic Acid (EMD Biosciences, San Diego, CA), 10 ng/ml Activin-A (R&D Systems, Minneapolis, MN), and 50 ng/ml recombinant human BMP-7 (R&D Systems) as previously described [ 9 ]. .. 64 Cu-PTSM Radiolabeling hES cells expressing firefly luciferase were dissociated using trypsin–EDTA (Invitrogen), counted, and plated in a 96-well plate in triplicate at 1.0×10 5 , 5.0×10 4 , 2.5×10 4 , 1.3×10 4 , 6.3×10 3 , 3.1×10 3 , and 1.6×10 3 cells per well.

    Derivative Assay:

    Article Title: Effect of low-intensity pulsed ultrasound on bone morphogenetic protein 7-induced osteogenic differentiation of human nonunion tissue-derived cells in vitro.
    Article Snippet: lthough fracture healing is uneventful in most cases, 5% to 10% of fractures fail to heal and result in a delayed union or persistent nonunion.1,2 Autogenous bone grafting has been the standard technique for enhancing nonunion healing for many years.3 However, bone grafting has drawbacks, such as the need for an additional surgical site with its associated perioperative morbidity (eg, pain, potential infection, blood loss, and fracture),4 and limits exist with respect to the size, shape, and quantity of bone grafts available.. Other treatment techniques have thus been developed.. Biological Takaaki Koga, MD, Sang Yang Lee, MD, PhD, Takahiro Niikura, MD, PhD, Akihiro Koh, MD, PhD, Yoshihiro Dogaki, MD, Etsuko Okumachi, MD, Toshihiro Akisue, MD, PhD, Ryosuke Kuroda, MD, PhD, Masahiro Kurosaka, MD, PhD

    Knock-Out:

    Article Title: Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology
    Article Snippet: .. ODM was composed of Minimum Essential Medium α (ThermoFisher Scientific, cat# 12571063) supplemented with 10% KnockOut Serum Replacement (ThermoFisher Scientific, cat# 10828028), hepatocyte growth factor (5 ng/mL) (R&D Systems, cat# 294-HG), activin A (100 ng/mL) (Sigma-Aldrich, cat# a4941), follicle stimulating hormone (1 ng/mL) (Sigma-Aldrich, cat# F4021), luteinizing hormone (1 ng/mL) (Sigma-Aldrich, cat# L5259), testosterone (1 μM) (Steraloids, cat# A6950-000), recombinant human BMP-4 (20 ng/mL) (R&D Systems, cat# 314-BP), recombinant human BMP-7 (20 ng/mL) (R&D Systems, cat# 354-BP), 3,3’,5-triodo-L-thyronine sodium (2 ng/mL) (Sigma-Aldrich, cat# T6397), l-ascorbic acid-2-glucoside (1 mM) (Matrix Scientific, cat# 092375) and 1% Penicillin-Streptomycin ( , ). ..



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    <t>BMP7</t> is upregulated in a hypoxia-induced pulmonary hypertension model. A-B Hemodynamic assessment of RVSP in hypoxic PH mice versus normoxic controls ( n = 6). C-D Western blot analysis of BMP7 protein expression in lung tissues of mice from different groups ( n = 6). E Hypoxia-mediated pulmonary vascular remodeling demonstrated by H&E staining ( n = 6). Scale bar: 50 μm. F Immunofluorescence co-staining of BMP7 and α-SMA in lung section. Scale bar: 25 μm. G-J Western blot analysis of BMP7 protein expression in human and mouse PASMCs cultured under hypoxic conditions ( n = 4). K-L The expression and subcellular localization of BMP7 in hypoxic mPASMCs was detected by immunofluorescence ( n = 4). Scale bar: 100 μm. Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: Con (control), ns (not significant)
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    Image Search Results


    BMP7 is upregulated in a hypoxia-induced pulmonary hypertension model. A-B Hemodynamic assessment of RVSP in hypoxic PH mice versus normoxic controls ( n = 6). C-D Western blot analysis of BMP7 protein expression in lung tissues of mice from different groups ( n = 6). E Hypoxia-mediated pulmonary vascular remodeling demonstrated by H&E staining ( n = 6). Scale bar: 50 μm. F Immunofluorescence co-staining of BMP7 and α-SMA in lung section. Scale bar: 25 μm. G-J Western blot analysis of BMP7 protein expression in human and mouse PASMCs cultured under hypoxic conditions ( n = 4). K-L The expression and subcellular localization of BMP7 in hypoxic mPASMCs was detected by immunofluorescence ( n = 4). Scale bar: 100 μm. Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: Con (control), ns (not significant)

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: BMP7 is upregulated in a hypoxia-induced pulmonary hypertension model. A-B Hemodynamic assessment of RVSP in hypoxic PH mice versus normoxic controls ( n = 6). C-D Western blot analysis of BMP7 protein expression in lung tissues of mice from different groups ( n = 6). E Hypoxia-mediated pulmonary vascular remodeling demonstrated by H&E staining ( n = 6). Scale bar: 50 μm. F Immunofluorescence co-staining of BMP7 and α-SMA in lung section. Scale bar: 25 μm. G-J Western blot analysis of BMP7 protein expression in human and mouse PASMCs cultured under hypoxic conditions ( n = 4). K-L The expression and subcellular localization of BMP7 in hypoxic mPASMCs was detected by immunofluorescence ( n = 4). Scale bar: 100 μm. Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: Con (control), ns (not significant)

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: Western Blot, Expressing, Staining, Immunofluorescence, Cell Culture, Control

    BMP7 promotes the proliferation and cell cycle progression of PASMCs, while inhibiting apoptosis. A CCK-8 assay assessing BMP7-induced changes in cell viability after 48-hour treatment ( n = 7). B-C Western blot analysis and quantitative densitometry of PCNA expression in BMP7-treated PASMCs ( n = 7). D-E Immunofluorescence detection of Ki67 expression in BMP7-treated PASMCs ( n = 4). Scale bar: 100 μm. F-G Western blot evaluation of cell cycle regulators (cyclin A2, cyclin B2, cyclin D1) ( n = 7). H-I Flow cytometry analysis of cell cycle distribution showing BMP7-mediated increase in S + G2/M phase cells ( n = 4). J-K Western blot analysis and quantification of apoptosis-related proteins (BCL2, BAX, Caspase3) ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: BMP7 promotes the proliferation and cell cycle progression of PASMCs, while inhibiting apoptosis. A CCK-8 assay assessing BMP7-induced changes in cell viability after 48-hour treatment ( n = 7). B-C Western blot analysis and quantitative densitometry of PCNA expression in BMP7-treated PASMCs ( n = 7). D-E Immunofluorescence detection of Ki67 expression in BMP7-treated PASMCs ( n = 4). Scale bar: 100 μm. F-G Western blot evaluation of cell cycle regulators (cyclin A2, cyclin B2, cyclin D1) ( n = 7). H-I Flow cytometry analysis of cell cycle distribution showing BMP7-mediated increase in S + G2/M phase cells ( n = 4). J-K Western blot analysis and quantification of apoptosis-related proteins (BCL2, BAX, Caspase3) ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: CCK-8 Assay, Western Blot, Expressing, Immunofluorescence, Flow Cytometry

    DNMT1 is downregulated in hypoxic pulmonary hypertension and regulates BMP7 expression. A-D Detection of DNMT1, DNMT3a, and DNMT3b in human and mouse PASMCs cultured under hypoxic conditions for 24, 48 and 72 h respectively ( n = 4). E-F Western blot analysis of DNMT1, DNMT3a, and DNMT3b protein levels in lung tissues of mice from different groups ( n = 6). G qRT-PCR analysis of DNMT1 mRNA levels in lung tissues from the indicated groups ( n = 6). H Immunofluorescence analysis of DNMT1 (red) and α-SMA (green) expression and co-localization in lung tissues. White arrows in the merged images denote co-localization signals. Scale bar: 25 μm. I-J The expression and subcellular localization of DNMT1 in mPASMCs were detected by immunofluorescence ( n = 4). Scale bar: 100 μm. K qRT-PCR analysis of BMP7 mRNA in mPASMCs treated with 5-Aza ( n = 6). L-M Western blot detection of BMP7 protein in 5-Aza-treated mPASMCs ( n = 6). N The mRNA levels of BMP7 after DNMT1 knockdown were measured by qRT-PCR ( n = 6). O-P BMP7 protein levels after DNMT1 knockdown were assessed by Western blot ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: Con (control), ns (not significant), 5-Aza (5-Azacytidine)

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: DNMT1 is downregulated in hypoxic pulmonary hypertension and regulates BMP7 expression. A-D Detection of DNMT1, DNMT3a, and DNMT3b in human and mouse PASMCs cultured under hypoxic conditions for 24, 48 and 72 h respectively ( n = 4). E-F Western blot analysis of DNMT1, DNMT3a, and DNMT3b protein levels in lung tissues of mice from different groups ( n = 6). G qRT-PCR analysis of DNMT1 mRNA levels in lung tissues from the indicated groups ( n = 6). H Immunofluorescence analysis of DNMT1 (red) and α-SMA (green) expression and co-localization in lung tissues. White arrows in the merged images denote co-localization signals. Scale bar: 25 μm. I-J The expression and subcellular localization of DNMT1 in mPASMCs were detected by immunofluorescence ( n = 4). Scale bar: 100 μm. K qRT-PCR analysis of BMP7 mRNA in mPASMCs treated with 5-Aza ( n = 6). L-M Western blot detection of BMP7 protein in 5-Aza-treated mPASMCs ( n = 6). N The mRNA levels of BMP7 after DNMT1 knockdown were measured by qRT-PCR ( n = 6). O-P BMP7 protein levels after DNMT1 knockdown were assessed by Western blot ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: Con (control), ns (not significant), 5-Aza (5-Azacytidine)

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: Expressing, Cell Culture, Western Blot, Quantitative RT-PCR, Immunofluorescence, Knockdown, Control

    DNMT1 inhibits PASMCs proliferation and cell cycle progression by downregulating BMP7. A-B Western blot validation of DNMT1 overexpression efficiency in PASMCs and its regulation of BMP7 expression ( n = 6). C CCK-8 assay assessing PASMCs viability under hypoxia post-DNMT1 overexpression or rBMP7 treatment ( n = 6). D-E Western blot analysis of PCNA expression in PASMCs under hypoxia following DNMT1 overexpression or rBMP7 treatment ( n = 6). F-G Immunofluorescence detection of Ki67 in PASMCs under hypoxic conditions following DNMT1 overexpression or rBMP7 treatment ( n = 6). Scale bar: 100 μm. H-I Western blot quantification of cyclin A2/B2/D1 in PASMCs under hypoxic conditions after DNMT1 overexpression or rBMP7 treatment ( n = 6). J-K Flow cytometry analysis of cell cycle distribution in PASMCs under hypoxic conditions following DNMT1 overexpression or rBMP7 treatment. ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: oeDNMT1 (overexpression of DNMT1), rBMP7 (recombinant BMP7)

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: DNMT1 inhibits PASMCs proliferation and cell cycle progression by downregulating BMP7. A-B Western blot validation of DNMT1 overexpression efficiency in PASMCs and its regulation of BMP7 expression ( n = 6). C CCK-8 assay assessing PASMCs viability under hypoxia post-DNMT1 overexpression or rBMP7 treatment ( n = 6). D-E Western blot analysis of PCNA expression in PASMCs under hypoxia following DNMT1 overexpression or rBMP7 treatment ( n = 6). F-G Immunofluorescence detection of Ki67 in PASMCs under hypoxic conditions following DNMT1 overexpression or rBMP7 treatment ( n = 6). Scale bar: 100 μm. H-I Western blot quantification of cyclin A2/B2/D1 in PASMCs under hypoxic conditions after DNMT1 overexpression or rBMP7 treatment ( n = 6). J-K Flow cytometry analysis of cell cycle distribution in PASMCs under hypoxic conditions following DNMT1 overexpression or rBMP7 treatment. ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: oeDNMT1 (overexpression of DNMT1), rBMP7 (recombinant BMP7)

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: Western Blot, Biomarker Discovery, Over Expression, Expressing, CCK-8 Assay, Immunofluorescence, Flow Cytometry, Recombinant

    DNMT1 binds to the BMP7 promoter and regulates the methylation of its CpG island. A Schematic diagram of the two CpG islands (CGI-1: c172,782,591 − 172,782,443, chromosome 2, GRCm39; CGI-2: c172,782,429 − 172,782,072, chromosome 2, GRCm39) identified in the BMP7 promoter region by MethPrimer. B-C Agarose gel electrophoresis of MSP products from bisulfite-converted DNA from mPASMCs or lung tissues in different group. Semi-quantitative analysis of methylation status is presented in the lower panels (M, methylated; U, unmethylated; n = 4). D ChIP-qPCR detected the binding of DNMT1 in both CpG islands and non-CpG island ( n = 6). E ChIP-qPCR assessed the effect of hypoxia on DNMT1 binding to the BMP7 promoter region ( n = 6). F-G Agarose gel electrophoresis of MSP products from bisulfite-converted DNA from mPASMCs treated with 5-Aza or siRNA. Semi-quantitative analysis of methylation status is presented in the lower panels ( n = 4). H Agarose gel electrophoresis of MSP products from bisulfite-converted DNA from mPASMCs overexpressing DNMT1. Semi-quantitative analysis of methylation status is presented in the lower panels ( n = 4). I ChIP-qPCR assessed the effect of DNMT1 overexpression on DNMT1 binding to the BMP7 promoter region ( n = 6). J-K qMSP analysis of BMP7 promoter methylation in hypoxia-treated mPASMCs and in mPASMCs upon DNMT1 knockdown ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: 5-Aza (5-Azacytidine), CpG island (CGI)

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: DNMT1 binds to the BMP7 promoter and regulates the methylation of its CpG island. A Schematic diagram of the two CpG islands (CGI-1: c172,782,591 − 172,782,443, chromosome 2, GRCm39; CGI-2: c172,782,429 − 172,782,072, chromosome 2, GRCm39) identified in the BMP7 promoter region by MethPrimer. B-C Agarose gel electrophoresis of MSP products from bisulfite-converted DNA from mPASMCs or lung tissues in different group. Semi-quantitative analysis of methylation status is presented in the lower panels (M, methylated; U, unmethylated; n = 4). D ChIP-qPCR detected the binding of DNMT1 in both CpG islands and non-CpG island ( n = 6). E ChIP-qPCR assessed the effect of hypoxia on DNMT1 binding to the BMP7 promoter region ( n = 6). F-G Agarose gel electrophoresis of MSP products from bisulfite-converted DNA from mPASMCs treated with 5-Aza or siRNA. Semi-quantitative analysis of methylation status is presented in the lower panels ( n = 4). H Agarose gel electrophoresis of MSP products from bisulfite-converted DNA from mPASMCs overexpressing DNMT1. Semi-quantitative analysis of methylation status is presented in the lower panels ( n = 4). I ChIP-qPCR assessed the effect of DNMT1 overexpression on DNMT1 binding to the BMP7 promoter region ( n = 6). J-K qMSP analysis of BMP7 promoter methylation in hypoxia-treated mPASMCs and in mPASMCs upon DNMT1 knockdown ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01. Abbreviations: 5-Aza (5-Azacytidine), CpG island (CGI)

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: Methylation, Agarose Gel Electrophoresis, ChIP-qPCR, Binding Assay, Over Expression, Knockdown

    Knockdown of BMP7 ameliorates hypoxia-induced pulmonary hypertension in vivo. A-B RVSP measurements and representative traces from mice in the indicated groups ( n = 6). C Quantification of right ventricular hypertrophy, presented as the ratio of right ventricular weight to the sum of left ventricular and septal weights [RV/(LV + S)] ( n = 6). D-F Echocardiographic analyses of PAVTI and the PAT/PET ratio were performed on the different groups ( n = 6). G-H Representative H&E-stained lung sections and quantitative analysis of vascular wall thickness ( n = 6). Scale bar: 100 μm. I-J Western blot analysis and quantification of PCNA protein expression in lung tissues ( n = 6). K-L Western blot quantification of cyclin A2/B2/D1 in lung tissues from different experimental groups ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: Knockdown of BMP7 ameliorates hypoxia-induced pulmonary hypertension in vivo. A-B RVSP measurements and representative traces from mice in the indicated groups ( n = 6). C Quantification of right ventricular hypertrophy, presented as the ratio of right ventricular weight to the sum of left ventricular and septal weights [RV/(LV + S)] ( n = 6). D-F Echocardiographic analyses of PAVTI and the PAT/PET ratio were performed on the different groups ( n = 6). G-H Representative H&E-stained lung sections and quantitative analysis of vascular wall thickness ( n = 6). Scale bar: 100 μm. I-J Western blot analysis and quantification of PCNA protein expression in lung tissues ( n = 6). K-L Western blot quantification of cyclin A2/B2/D1 in lung tissues from different experimental groups ( n = 6). Data are depicted as scatter plots with the median and interquartile range superimposed. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: Knockdown, In Vivo, Staining, Western Blot, Expressing

    Schematic of the mechanism. Decreased DNMT1 expression during hypoxia leads to hypomethylation of CpG islands in the BMP7 promoter region, which in turn leads to increased BMP7 expression and promotes PASMCs proliferation and pulmonary vascular remodeling

    Journal: Respiratory Research

    Article Title: DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7

    doi: 10.1186/s12931-025-03436-x

    Figure Lengend Snippet: Schematic of the mechanism. Decreased DNMT1 expression during hypoxia leads to hypomethylation of CpG islands in the BMP7 promoter region, which in turn leads to increased BMP7 expression and promotes PASMCs proliferation and pulmonary vascular remodeling

    Article Snippet: Primary human PASMCs (hPASMCs; Sciencell #3110) and murine PASMCs (mPASMCs; Pricella) were cultured in smooth muscle cell medium (SMCM; Sciencell #1101) supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, where all cellular assays including proliferation measurements were conducted under these standard serum-supplemented conditions; for hypoxic exposure, cells were incubated in a specialized atmosphere comprising 3% O2, 5% CO2, and 92% N2 for the indicated durations, while for pharmacological treatments, recombinant human BMP7 protein (5666-BP, R&D Systems) was administered at a concentration of 100 ng/mL for 48 h and the DNMT inhibitor 5-Azacytidine (5-Aza; ab142744, Abcam) was applied at 2 μM for 48 h, with all experiments using cells between passages 2–4 to ensure consistency.

    Techniques: Expressing